Figure 3. Serial 5 µm Z-axis planes and deconvolution of images taken with the appropriate filters for visualizing 5HT/AMCA, GAD/FITC and TH/TRITC: 1, 2, 3: Above, in and below focus planes respectively. 4. Specific Z-axis plane corrected for the perspective background flares using a deconvolve subroutine. Bar = 10 µm.