Figure 1. Low-power, dark field photomicrographs of 5HT (left) and TH (right) immunoperoxidase-stained fibers in rat mPFCx. Cortical laminae are denoted by the Roman numerals on the left. Bar = 100 µm.

Figure 2. High-power, bright field photomicrographs of 5HT (A) and TH (B) immunoperoxidase-stained fibers in apposition with cresyl violet stained cell bodies in layer II of rat mPFCx. Arrows indicate varicosities in close apposition to cell bodies. Bar = 10 µm.

Figure 3. Serial 5 µm Z-axis planes and deconvolution of images taken with the appropriate filters for visualizing 5HT/AMCA, GAD/FITC and TH/TRITC: 1, 2, 3: Above, in and below focus planes respectively. 4. Specific Z-axis plane corrected for the perspective background flares using a deconvolve subroutine. Bar = 10 µm.

Figure 4. Co-registered images of 5HT-, GAD- and TH-IR staining: A, B, C: Three deconvolved images showing a convergence of monoaminergic inputs (arrows = 5HT; arrowheads = TH) to GABAergic cell bodies in rat mPFCx. D. Monoaminergic varicosities converge on a pyramidal neuron (P) receiving GAD-IR terminals in rat mPFC. There also appear to be appositions between GAD-IR terminals and 5HT-IR varicosities (*). Similar interactions also seem to occur between GAD-IR terminals and TH-IR varicosities. Bar = 10 µm.