Figure 2: A concentration response curve was generated for alpha1 or alpha2 subunit expressing oocytes. Oocytes were perfused with glycine (5-1000uM) for 30 sec. Values are expressed as a percent of the maximal glycine (500 uM) response (n=5-15). An EC50 of 85 uM and a Hill coefficient of 2.5 were calculated for alpha1 subunit receptors, and an EC50 of 142 uM and a Hill coefficient of 2.29 were calculated for alpha2 subunit receptors, respectively.