Figure 1:This figure shows a rat Purkinje cell in primary culture. Subsequent to loading with Fura-2AM (3 uM for 25 min in 5% CO₂ at 36 degrees C.), the culture was equilibrated at room temperature for 35 minutes in artificial CSF. The culture was then utilized for calcium imaging. Relative intensity of intracellular calcium is indicated on the vertical scale bar, with red to white indicating the highest concentration (approximately 1 uM). The horizontal scale bar=50 um. Used with permission from Howard Strahlendorf, 1996.