Figure 2:(top) This figure is a magnification of the extensive, probably axonal, process in Figure 1. This frame is taken subsequent to glutamate administration (10 uM). Note red calcium "hot spots" along the process, in response to glutamate-induced depolarization. (bottom) Same process, 50 msec later. Note disappearance of "hot spots". Vertical intensity scale bar as in Figure 1. Horizontal scale bar=25 um.